E-MEXP-3885 - Transcription profiling by array of Arabidopsis thaliana wild type and atglr3.3 mutant seedlings treated with CK buffer containing GSH and Glu
Released on 30 September 2013, last updated on 3 June 2014
Arabidopsis wild type and atglr3.3 mutant seedlings were cultured on 0.7% (w/v) agar medium containing half strength of MS basic salt with 1% sucrose (w/v), 5mM MES, pH 5.7. 10-day-old seedlings were equilibrated in the CK buffer for 24 hours. Then, these seedlings were either treated with the CK buffer or the CK buffer containing 100 ?M GSH and 100 ?M Glu for 1 hour, respectively.
transcription profiling by array, co-expression, compound treatment design, replicate design
Glutamate Receptor Like Channel 3.3 is involved in mediating glutathione-triggered cytosolic Ca2+ transients, transcriptional changes and innate immunity responses in Arabidopsis.