E-MEXP-3883 - Transcription profiling by array of mouse neural progenitor cells isolated from forebrains of E13.5 dpc mouse embryos with wild-type Miz1 or homozygous for Miz1-delta-POZ
Released on 1 October 2013, last updated on 3 June 2014
To isolate neuronal progenitor cells (NPCs), forebrains of E13.5 Miz1+/+ or Miz1-delta-POZ embryos were cut in small pieces, digested with trypsin and filtered through sterile gauze. Cells were cultivated in 2:1 DMEM/F12 supplemented with 1xB27 (Life technologies), 20 ng/ul EGF (Biomol), 20 ng/?l basic FGF (Biomol), 1 ug/ml fungizone (Gibco) and Penicillin/Streptomycin (PAA). NPCs were passaged every seven days. RNA expression of different genotypes was compared in sec. and quart. neurospheres.
transcription profiling by array, co-expression, genetic modification design, in vitro, time series design
Miz1 is required to maintain autophagic flux. Wolf E, Gebhardt A, Kawauchi D, Walz S, von Eyss B, Wagner N, Renninger C, Krohne G, Asan E, Roussel MF, Eilers M. :2535 (2013), PMID:24088869
Miz1 is required to maintain autophagic flux. Elmar Wolf,Anneli Gebhardt,Daisuke Kawauchi,Susanne Walz,Björn von Eyss,Nicole Wagner,Christoph Renninger,Georg Krohne,Esther Asan,Martine F Roussel,Martin Eilers. Nat Commun (2013)