E-MEXP-3870 - Transcription profiling by array of individual human oocytes and embryos
Released on 13 December 2013, last updated on 3 May 2014
Early development in humans is characterised by low and variable embryonic viability, reflected in low fecundity and high rates of miscarriage, relative to other mammals. Data from assisted reproduction programmes provides additional evidence that this is largely mediated at the level of embryonic competence and is highly heterogeneous among embryos. Understanding the basis of this heterogeneity has important implications in a number of areas including: the regulation of early human development, disorders of pregnancy, assisted reproduction programmes, the long term health of children which may be programmed in early development, and the molecular basis of pluripotency in human stem cell populations. We have therefore investigated global gene expression profiles using polyAPCR amplification and microarray technology applied to individual human oocytes and 4-cell and blastocyst stage embryos. In order to explore the basis of any variability in detail, each developmental stage is replicated in triplicate. Our data show that although transcript profiles are highly stage-specific, within each stage they are relatively variable.
transcription profiling by array, co-expression, development or differentiation design, in vivo
Global gene expression profiling of individual human oocytes and embryos demonstrates heterogeneity in early development. Shaw L, Sneddon SF, Zeef L, Kimber SJ, Brison DR. PLoS One 8(5):e64192 (2013), PMID:23717564