8 protocols
AccessionType
image_acquisition
The scanning was performed according to the manufacturers protocol (Agilent technologies, A Method for Quantifying the performance of a DNA Microarray Scanner, Publication Number 5988-9498EN). (Parameters: Scanning hardware = DNA Microarray Scanner BA [Agilent Technologies], Scanning software = Feature Extraction Software [Agilent])
hybridization
The hybridization experiment was performed according to the manufacturers protocol (Agilent technologies, Agilent 60-mer oligo microarray processing protocol V2.1 (SSPE wash/6-screw Hyb Chamber), Publication Number G4140-90055V2.1APRIL2004). (Parameters: Chamber type = OTHER: Agilent-Microarray hybridization chamber, Quantity of label target used = 0.5, Mass unit = Micro gram, Tiny time unit = seconds, Volume unit = Nano litre, temperature = 60)
labeling
The labeling experiment was performed according to the manufacturers protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
labeling
The labeling experiment was performed according to the manufacturers protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1). (Parameters: Amplification = none, Mass unit = Micro gram)
specified_biomaterial_action
The plants were incubated at 28C under non-stress condition.
nucleic_acid_extraction
Total RNAs were isolated using the RNAiso Reagent (Takara, Japan). (Parameters: Extracted product = total_RNA, Amplification = none)
grow
Seeds of rice (Oryza sativa var. Nipponbare) were grown under controlled conditions, using 28C day/25C night temperatures, 12-h light/12-h dark cycles, and 80±5% relative humidity in nutrient soil. (Parameters: start time = 2, time unit = weeks, min temperature = 28, temperature unit = C, media = soil)
specified_biomaterial_action
Two-week-old plants were incubated for 2 days without watering. The soil moisture content was 18.7% on day 2 of dehydration.