E-MEXP-3852 - Transcription profiling by array of Pleuronichthys verticalis (Hornyhead turbot) captured from wastewater treatment plant discharge locations to investigate endocrine disruption
Released on 10 December 2013, last updated on 15 January 2015
Hornyhead turbot (Pleuronichthys verticalis) captured near sewage outfalls are used as sentinel fish for monitoring exposure to industrial and agricultural chemicals of ~20 million people living in coastal southern California. Although analyses of hormones in blood and organ morphology and histology in fish are useful for assessing exposure, there is a need for quantitative and sensitive molecular measurements, as many contaminants produce subtle effects. A novel multispecies microarray and qRT-PCR were used to investigate endocrine disruption in turbot captured near sewage outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g. estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with phenotypic end points.
transcription profiling by array, co-expression, reference design, replicate design, stimulus or stress design
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. Baker ME, Vidal-Dorsch DE, Ribecco C, Sprague LJ, Angert M, Lekmine N, Ludka C, Martella A, Ricciardelli E, Bay SM, Gully JR, Kelley KM, Schlenk D, Carnevali O, Sášik R, Hardiman G. PloS one 8(9):e75553 (2013), Europe PMC 24086568