E-MEXP-3805 - Transcription profiling by array of human monocyte-derived macrophages infected with Mycobacterium tuberculosis (H37Rv strain)
Released on 1 June 2013, last updated on 3 May 2014
Human monocyte-derived macrophages were infected with Mycobacterium tuberculosis (H37Rv strain) at a multiplicity of infection 1:1. After 2h of incubation, cells were washed to remove extracellular bacteria and were incubated in fresh medium for a further 18h in the presence or not of 100microM ATP. Total RNA was then extracted and gene expression was determined using HuGene 1.0 ST microarrays (Affymetrix, Inc.,Santa Clara, CA).
transcription profiling by array, co-expression, compound treatment, disease state design, in vitro