Title: Affymetrix CEL analysis. Description:
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
To prepare total RNA for microarray analysis, we used retinas from 4 month-old Wt and TgIGF-I mice. We used the recommended Affymetrix protocol for RNA extraction (Expression Analysis Technical Manual; http://www.affymetrix.com). Briefly, each pair of retinas were homogenised with Trizol (Invitrogen) before precipitation and column purification (Qiagen). On-column DNase I digestion was carried out (Qiagen).
(Parameters: Extracted product = total RNA, Amplification = none)