5 protocols
Title: Affymetrix CEL analysis. Description:
hybridization protocol
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
To prepare total RNA for microarray analysis, we used retinas from 4 month-old Wt and TgIGF-I mice. We used the recommended Affymetrix protocol for RNA extraction (Expression Analysis Technical Manual; http://www.affymetrix.com). Briefly, each pair of retinas were homogenised with Trizol (Invitrogen) before precipitation and column purification (Qiagen). On-column DNase I digestion was carried out (Qiagen).
(Parameters: Extracted product = total RNA, Amplification = none)