7 protocols
AccessionType
feature_extraction
Title: Affymetrix CEL analysis. Description:
labeling
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended labeling protocols for use with GeneChip Exon Arrays.
hybridization
URL: http://www.affymetrix.com/support/technical/manual.affx
Affy_whole_transcript_labelling.pdf
This document describes Affymetrix recommended hybridization protocols for use with GeneChip Exon Arrays.
nucleic_acid_extraction
Total RNA of each mouse liver was extracted by using TRIzol reagent (Invitrogen, CA, USA) and purified by Qiagen RNeasy MiniRNA Cleanup Kit (Qiagen, Valencia, CA, USA) according to the recommended protocol.
(Parameters: Extracted product = total_RNA, Amplification = none)
specified_biomaterial_action
Mice were scarified by eyeball blooding at the end of trial, and then the livers were quickly removed, cut into pieces and finally stored in different preserving liquids for the following experiments.
pool
Small pieces of mice livers weighting 0.5 g each were pooled to generate mixture for RNA extraction
grow
All the mice were randomly divided into four groups and placed in individual stainless steel cages under controlled conditions (22 ᄆ 2 ᄚC, 50 ᄆ 5% humidity, 12/12 light dark cycle). Purified water and contaminants along with standard mouse food (TROPHIC Co., Ltd., Nantong, China) were fed ad libitum to the four groups for 90 days.
(Parameters: start time = 90, time unit = days, min temperature = 22, temperature unit = C, media = Mus musculus)