Title: Affymetrix CEL analysis. Description:
This document describes Affymetrix recommended labeling protocols for use with GeneChip Exon Arrays.
This document describes Affymetrix recommended hybridization protocols for use with GeneChip Exon Arrays.
Total RNA of each mouse liver was extracted by using TRIzol reagent (Invitrogen, CA, USA) and purified by Qiagen RNeasy MiniRNA Cleanup Kit (Qiagen, Valencia, CA, USA) according to the recommended protocol.
(Parameters: Extracted product = total_RNA, Amplification = none)
Mice were scarified by eyeball blooding at the end of trial, and then the livers were quickly removed, cut into pieces and finally stored in different preserving liquids for the following experiments.
Small pieces of mice livers weighting 0.5 g each were pooled to generate mixture for RNA extraction
All the mice were randomly divided into four groups and placed in individual stainless steel cages under controlled conditions (22 ﾱ 2 ﾰC, 50 ﾱ 5% humidity, 12/12 light dark cycle). Purified water and contaminants along with standard mouse food (TROPHIC Co., Ltd., Nantong, China) were fed ad libitum to the four groups for 90 days.
(Parameters: start time = 90, time unit = days, min temperature = 22, temperature unit = C, media = Mus musculus)