5 protocols
Title: Affymetrix CEL analysis. Description:
hybridization protocol
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
Sorted cells were lysed were lysed in RLT buffer (Qiagen) and further processed on Qiagen Rneasy Micro columns including DNAse treatment (Parameters: Extracted product = total_RNA, Amplification = none)
GVHD was induced in recipients, using a haploidentical P into F1 murine transplantation model. Some recipients were injected i.p. peritransplantally with 5mg/kg/day Fgf7 for 7 days. Control group received HBSS. Recipient's thymi were dissected, digested with Collagenase/Dispase/Dnase and thymic epithelial cells were sorted by FACS.