5 protocols
AccessionType
feature_extraction
Title: Affymetrix CEL analysis. Description:
hybridization protocol
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic_acid_extraction
Sorted cells were lysed were lysed in RLT buffer (Qiagen) and further processed on Qiagen Rneasy Micro columns including DNAse treatment (Parameters: Extracted product = total_RNA, Amplification = none)
specified_biomaterial_action
GVHD was induced in recipients, using a haploidentical P into F1 murine transplantation model. Some recipients were injected i.p. peritransplantally with 5mg/kg/day Fgf7 for 7 days. Control group received HBSS. Recipient's thymi were dissected, digested with Collagenase/Dispase/Dnase and thymic epithelial cells were sorted by FACS.