5 protocols
AccessionType
image_acquisition
Affymetrix Scanning protocol
(Parameters: Scanning hardware = 418 [Affymetrix], Scanning software = MAS/GCOS/GREX [Affymetrix])
hybridization
Hybridization
(Parameters: Chamber type = Affymetrix- GeneChip Hyb Oven 640, Quantity of label target used = 12.5, Mass unit = Micro gram, Tiny time unit = seconds, Volume unit = Nano litre, temperature = 45)
labeling
Affymetrix IVT protocol
(Parameters: Amplification = none, Mass unit = Micro gram)
nucleic_acid_extraction
Total RNA was extracted from frozen liver tissue by using RNeasy Mini kit (Qiagen)
(Parameters: Extracted product = total_RNA, Amplification = none)
specified_biomaterial_action
Cells were treated prior to extraction.