2 protocols
Total bacterial RNA was isolated using Trizol according to the manufacturer's recommendations (Invitrogen) (Parameters: Extracted product = total_RNA, Amplification = none)
Both strains were grown on Schaedler blood agar (SBA) plates (Schaedler-agar supplemented with 5% blood [Quad Five, Ryegate, MT]) at 37C and 5% CO2 for 48 hours. The cells were resuspended in Gerhardt’s Minimal Media and exposed to 5mM H2O2(hydrogen peroxide). Cultures were grown at 37C and shaking for 10 min at which time the cells were harvested for RNA isolation. (Parameters: start time = 60, time unit = minutes, min temperature = 37, temperature unit = C, media = Gerhardt’s Minimal Media)