E-MEXP-3707 - Transcription profiling of mouse livers which have been exposed to dechlorane plus by gavage

Status
Released on 5 September 2012, last updated on 29 October 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (4)
Description
Five-week-old male mice (Mus musculus, ICR) were exposed to dechlorane plus for 10 days. A total of tweenty-four mice were randomly assigned to control and three DP-treated groups. Six mice were applied in every group. For control group, corn oil was given to mice by gavage daily. For three DP-treated groups, mice received a daily dose of DP in corn oil at 500 mg DP /kg bw, 2000 mg DP /kg bw and 5000 mg DP /kg bw, respectively. After exposure, mice were anaesthetized under isoflurane followed by exsanguination. Livers were removed, and hepatic RAN for each mouse were immediately extract. The gene expression profiles for control and three DP-treated groups were determined by the GeneChip? Mouse Exon 1.0 ST arrays. the biological significances of differentially expressed genes (DEGs) were analyzed based on Molecule Annotation System 3.0 (MAS3.0, http://bioinfo.capitalbio.com/mas/). The microarray analysis of this study provide novel insight regarding toxicological effects and mechanism of DP at the transcriptome level
Experiment types
transcription profiling by array, co-expression, dose response, in vivo
Contact
Citation
Responses of Mouse Liver to Dechlorane Plus Exposure by Integrative Transcriptomic and Metabonomic Studies. Bing Wu, Su Liu, Xuechao Guo, Yan Zhang, Xuxiang Zhang, Mei Li, Shupei Cheng. Environ. Sci. Technol. 46(19):10758–10764 (2012), Europe PMC 22913625
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3707.idf.txt
Sample and data relationshipE-MEXP-3707.sdrf.txt
Raw data (1)E-MEXP-3707.raw.1.zip
Additional data (1)E-MEXP-3707.additional.1.zip
Array designA-AFFY-130.adf.txt
Links