2 protocols
Total RNA was isolated from both mock infected and virus infected HT29 cells using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and purified by of RNeasy Mini Kit (Qiagen, Germany). RNA quality was assessed by Agilent 2100 bioanalyser using the RNA 6000 Nano Chip (Agilent Technologies), and quantity was determined by Nanodrop Spectrophotometer (Eppendorf, Germany)
(Parameters: Extracted product = total_RNA, Amplification = none)
At first three rota virus strains(SA11, Wa and A5-13) were cultured in MA104 cell. After 24 hours of post infection, viruses were isolated and purified. Then HT29 cells either mock infected or infected with SA11 or A5-13 or Wa strain of rota virus.