3 protocols
Total RNA integrity was evaluated using the Agilent 2100 bioanalyzer. RNAs were then labeled with the Quick Amp Labeling Kit, one-color as suggested by the manufacturer
(Parameters: Amount of nucleic acid labeled = 200, Amplification = none, Mass unit = Nano gram)
Cells were treated by 1µM atRA for 1 hour or with DMSO 0.1% for the same duration. Total RNAs were extracted and processed
Total RNAs were extracted by the Trizol method
(Parameters: Extracted product = total_RNA, Amplification = none)