E-MEXP-3667 - Transcription profiling of a yeast protein kinase PKC1 temperature sensitive mutant on cells synchronised in S phase and then released
Released on 14 March 2013, last updated on 3 May 2014
Transcriptional profiling of a Saccharomyces cerevisiae protein kinase Pkc1p temperature sensitive mutant on cells synchronised in S phase and then released. The Mcm1p-Fkh2p-Ndd1p complex in Saccharomyces cerevisiae controls the cyclical expression of the CLB2 cluster of genes at the G2/M phase transition. The activity of this complex is known to be positively controlled by cyclin-CDK and polo kinases. The current experiment aimed to demonstrate that the protein kinase Pkc1p works in the opposite manner to inhibit the activity of the Mcm1p-Fkh2p-Ndd1p complex and the expression of its target genes.
transcription profiling by array, cell cycle, co-expression, in vivo
Protein Kinase C Regulates Late Cell Cycle-Dependent Gene Expression. Zoulfia Darieva; Namshik Han; Stacey Warwood; Kathryn S. Doris; Brian A. Morgan; Andrew D. Sharrocks. Mol Cell Biol 32(22):4651 (2012), Europe PMC 22966207