E-MEXP-3667 - Transcription profiling of a yeast protein kinase PKC1 temperature sensitive mutant on cells synchronised in S phase and then released

Released on 14 March 2013, last updated on 3 May 2014
Saccharomyces cerevisiae
Samples (8)
Array (1)
Protocols (4)
Transcriptional profiling of a Saccharomyces cerevisiae protein kinase Pkc1p temperature sensitive mutant on cells synchronised in S phase and then released. The Mcm1p-Fkh2p-Ndd1p complex in Saccharomyces cerevisiae controls the cyclical expression of the CLB2 cluster of genes at the G2/M phase transition. The activity of this complex is known to be positively controlled by cyclin-CDK and polo kinases. The current experiment aimed to demonstrate that the protein kinase Pkc1p works in the opposite manner to inhibit the activity of the Mcm1p-Fkh2p-Ndd1p complex and the expression of its target genes.
Experiment types
transcription profiling by array, cell cycle, co-expression, in vivo
Protein Kinase C Regulates Late Cell Cycle-Dependent Gene Expression. Zoulfia Darieva; Namshik Han; Stacey Warwood; Kathryn S. Doris; Brian A. Morgan; Andrew D. Sharrocks. Mol Cell Biol 32(22):4651 (2012), Europe PMC 22966207
Investigation descriptionE-MEXP-3667.idf.txt
Sample and data relationshipE-MEXP-3667.sdrf.txt
Raw data (1)E-MEXP-3667.raw.1.zip
Processed data (1)E-MEXP-3667.processed.1.zip
Array designA-AFFY-47.adf.txt