2 protocols
Expression data was read from raw cel files using the ReadyAffy function in the affy package in R version 2.15.0. Data was annotated using Bioconductor annotation libraries. Data was normalised using the Robust Multi-Array Average expression measure (rma) function in the affya package, transforming expression data into normalised log 2 intensity values for further analysis.
Total RNA was isolated using Trizol reagent according to the manufactureres instructions (Invitrogen) (Parameters: Extracted product = total_RNA, Amplification = none)