4 protocols
nucleic acid extraction protocol
Trizol extraction
(Parameters: Extracted product = genomic_DNA, Amplification = none)
RMA using the Affy expression console
Affy expression console (Parameters: Scanning hardware = GeneChip Scanner 3000 [Affymetrix], Scanning software = Scanning software)
We overexpressed N2ICD specifically in the myocardium by crossing ?MHC (alpha-myosin heavy chain) - Cre mice with RosaN2ICD mice, which carry a modified Rosa26 locus targeted with a constitutively active form of Notch2 preceded by a floxed STOP cassette. The generation of RosaN2ICD mice were described previously in: Tschorz, JS et al Hepatology 2009 Sep, 50(3):871-9.