E-MEXP-3576 - Comparative genomic hybridization by array of yeast to to analyse aneuploidy in mutant strains (ies6d and ino80d) compared to wild-type over time
Last updated on 15 May 2013, released on 16 May 2013
Genomic DNA was collected from ies6d or ino80d after undergoing 40 or 80 generations post-sporulation of IES6/ies6 or 20 or 40 generations post-sporulation of INO80/ino80 heterozygous diploid strains, respectively. (1 day = 10 generations.) Genomic DNA was then amplified, and hybridized to a whole genome tiling microarray. Copy number variation was measured relative to a G1 arrested wild type sample.
comparative genomic hybridization by array, comparative genome hybridization, genetic modification, in vivo, strain or line, time series
The INO80 chromatin remodeling complex prevents polyploidy and maintains normal chromatin structure at centromeres. Chambers AL, Ormerod G, Durley SC, Sing TL, Brown GW, Kent NA, Downs JA. Genes & Development :2590–2603 (2012)