E-MEXP-3437 - Transcription profiling by array of Candida albicans cells treated with antifungal aminocandin versus untreated cells

Status
Released on 1 March 2012, last updated on 2 May 2014
Organism
Candida albicans
Samples (4)
Array (1)
Protocols (5)
Description
We found that the antibiotic colistin acts synergistically with antifungals of the echinocandin class (e.g. aminocandin) on C. albicans cells. In order to elucidate the mode of action of colistin in fungi we performed microarray analysis of samples treated with only aminocandin (0.00125µg/ml) or treated with aminocandin (0.00125µg/ml) and colistin (5µg/ml). We compared: (A). untreated cells to cells treated with aminocandin only; (B). cells treated with aminocandin to cells treated with aminocandin and colistin. By comparing those datasets it should be possible to identify genes differentially expressed in response to aminocandin and in response to both drugs. And subsequently to be able to interpret where in the cell colistin acts. (See related experiment in ArrayExpress: E-MEXP-3438)
Experiment types
transcription profiling by array, co-expression, compound treatment, dye swap, replicate
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3437.idf.txt
Sample and data relationshipE-MEXP-3437.sdrf.txt
Raw data (1)E-MEXP-3437.raw.1.zip
Array designA-MEXP-2142.adf.txt
Links