E-MEXP-3406 - Transcription profiling by array of miRNA from the spleen and intestine of germ free, specific pathogen free and IL-10 knockout mice

Status
Last updated on 23 November 2011, released on 23 November 2011
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (4)
Description
This experiment was investigating how gut commensal bacteria and intestinal inflammation affect miRNA expression. We analyzed miRNA expression of spleen and intestine from specific pathogen free (SPF) B6 mice, germ-free (GF) B6 mice, and IL-10 knockout mice which have severe colitis by microarray. Thus we have total 6 samples: GF spleen; GF intestines; SPF spleen; SPF intestine; IL-10 KO spleen and IL-10 KO intestine. We directly isolated RNA from whole spleens or intestines without any treatments, and then did microarray analysis.
Experiment types
transcription profiling by array, co-expression, ex vivo, physiological process
Contact
Citations
Microbiota Downregulates Dendritic Cell Expression of miR-10a, Which Targets IL-12/IL-23p40. Xue, Xiaochang; Feng, Ting; Yao, Suxia; Wolf, Kyle J.; Liu, Chang-Gong; Liu, Xiuping; Elson, Charles O.; Cong, Yingzi. J Immunol 187(11):5879 (2011), PMID:22068236
Microbiota downregulates dendritic cell expression of miR-10a, which targets IL-12/IL-23p40. Xue X, Feng T, Yao S, Wolf KJ, Liu CG, Liu X, Elson CO, Cong Y. :5879-5886 (2011), PMID:22068236
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3406.idf.txt
Sample and data relationshipE-MEXP-3406.sdrf.txt
Raw data (1)E-MEXP-3406.raw.1.zip
Array designA-MEXP-1838.adf.txt