E-MEXP-3406 - Transcription profiling by array of miRNA from the spleen and intestine of germ free, specific pathogen free and IL-10 knockout mice
Last updated on 23 November 2011, released on 23 November 2011
This experiment was investigating how gut commensal bacteria and intestinal inflammation affect miRNA expression. We analyzed miRNA expression of spleen and intestine from specific pathogen free (SPF) B6 mice, germ-free (GF) B6 mice, and IL-10 knockout mice which have severe colitis by microarray. Thus we have total 6 samples: GF spleen; GF intestines; SPF spleen; SPF intestine; IL-10 KO spleen and IL-10 KO intestine. We directly isolated RNA from whole spleens or intestines without any treatments, and then did microarray analysis.
transcription profiling by array, co-expression, ex vivo, physiological process
Microbiota Downregulates Dendritic Cell Expression of miR-10a, Which Targets IL-12/IL-23p40. Xue, Xiaochang; Feng, Ting; Yao, Suxia; Wolf, Kyle J.; Liu, Chang-Gong; Liu, Xiuping; Elson, Charles O.; Cong, Yingzi. J Immunol 187(11):5879 (2011), PMID:22068236
Microbiota downregulates dendritic cell expression of miR-10a, which targets IL-12/IL-23p40. Xue X, Feng T, Yao S, Wolf KJ, Liu CG, Liu X, Elson CO, Cong Y. :5879-5886 (2011), PMID:22068236