Title: Affymetrix CEL analysis. Description:
Affymetrix Generic Hybridization
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
Title: Affymetrix CHP Analysis (ExpressionCall). Description:
Total RNA including miRNA is extracted using qiagen miRNEasy mini Kit (Cat No. 217004) according to manufacturer's protocols. RNA quality was assessed by using the Agilent 2100 bioanalyzer and only samples with RNA integrity number >8 were used.
(Parameters: Extracted product = total_RNA, Amplification = none)
miR-21 wildtype or miR-21 deficient bone marrow derived progenitor cells were treated with 100 ng/mL stem cell factor and 100 ng/mL FLT-3 ligand from day 0 to day 4. On day 4, stem cell factor and FLT-3 ligand were replaced with 10 ng/mL IL-5 and the cells were cultured for an additional 10 days.