Title: Affymetrix CEL analysis. Description:
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
Total RNA was extracted from three-day-old whole seedlings using TRIzol (Invitrogen) and purified with an RNeasy microkit (QIAGEN) as described in the manufacturer’s instructions.
(Parameters: Extracted product = total_RNA, Amplification = none)
Arabidopsis plants were grown in Murashige and Skoog (MS) medium [1 x MS salts, 1 x MS vitamins, 2% (w/v) sucrose (pH 6.3), and 0.8% agar] under continuous light conditions at 23°C.
(Parameters: time unit = seconds, temperature unit = C)