E-MEXP-33 - Transcription profiling of aortic media from L-NAME and atorvastatin treated male rats

Released on 31 December 2006, last updated on 2 May 2014
Rattus norvegicus
Samples (42)
Arrays (3)
Protocols (16)
One-month-old male Fischer 344 rats with an initial body weight of 150 g were used for this study and were divided into 7 groups: a control group (30 days), 2 groups intoxicated with L-NAME (50 mg/kg per day in the drinking water; Sigma Chemical Co) for 15 and 30 days, 2 groups treated with atorvastatin (100 mg/kg per day in the drinking water; Pfizer France) for 15 and 30 days and 2 groups receiving L-NAME (50 mg/kg/day) and atorvastatin (100 mg/kg/day) for 15 and 30 days (6 rats in each group). We compared transcriptional profile of aortic medias of control and treated rats using the Affymetrix U34 set. For each a set (chips A, B and C), we pooled RNAs from two rats. The same labelled extract was used for chips A, B and then C. We used 3 independant pools of RNA from 2 rats per treatment group to obtain biological triplicates.
Experiment types
transcription profiling by array, compound treatment
Morgan Dupuis <arrayexpress@ebi.ac.uk>, Florent Soubrier, Frederique Capron, Isabelle Brocheriou, Jean-Baptiste Michel, Liliane Louedec, Mounsif Haloui, Segolene Raoux, Sophie Nadaud
counter-regulation by atorvastatin of gene modulations induced by L-NAME hypertension is associated with vascular protection. Morgan Dupuis; Sophie Nadaud; Isabelle Brocheriou; Mounsif Haloui; Liliane Louedec; Segolene Raoux; Frederique Capron; Jean-Baptiste Michel; Florent Soubrier.