E-MEXP-3204 - impact of polyphenols on miRNA expression in liver
Released on 13 April 2012, last updated on 13 May 2014
At eight weeks of age, mice were divided into 10 groups with six mice per group and fed either the control-diet or the same diet supplemented with quercetin, hesperidin, naringenin, anthocyanin, catechin and curcumin at 0.006% (w/w) corresponding to an equivalent intake in humans of 30 mg/day, while proanthocyanin, caffeic acid and ferulic acid were added at 0.06% (w/w) corresponding to an equivalent intake in humans of 300 mg/day. After two weeks of supplementation, mice were sacrificed under pentobarbital anesthesia. The organs were washed with physiological saline solution maintained at 37C by direct injection in the heart’s left ventricle. Livers were collected, immediately frozen in liquid nitrogen and stored at -80C until the time of analysis.
transcription profiling by array, co-expression, compound treatment, genetic modification, in vivo
Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols.