E-MEXP-3199 - ChIP-chip, RIP-chip and transcription profiling by array of human T-cells to identify the set of mRNAs that HIV-1 Tat interacts with in T-cells
Released on 5 November 2013, last updated on 23 July 2014
We have used RNA immunoprecipitation to identify the set of mRNAs that HIV-1 Tat interacts with in T-cells. We have also performed measurements of relative RNA abundance to determine if Tat binding is associated with an increase in RNA abundance in Tat-expressing T-cells and during HIV infection of primary T-cells. We have also used RNA IP and ChIP-Chip to compare the RNAs with which Tat interacts with to the RNAs that RISC interacts with and the genes associated with pTEF-b.
transcription profiling by array, ChIP-chip by array, RIP-chip by array, all pairs, binding site identification, co-expression, disease state
Human immunodeficiency virus Tat associates with a specific set of cellular RNAs. Bouwman RD, Palser A, Parry CM, Coulter E, Rasaiyaah J, Kellam P, Jenner RG. Retrovirology 11:53 (2014), Europe PMC 24990269