E-MEXP-3170 - CCCB - Genomic variation

Status
Released on 21 April 2011, last updated on 2 May 2014
Organism
Streptococcus pneumoniae
Samples (5)
Arrays (2)
Protocols (7)
Description
Antibiotic resistance in Streptococcus pneumoniae is often the result of horizontal gene transfer events involving closely related streptococcal species. Laboratory experiments confirmed that S. mitis DNA functions as donor in transformation experiments, using the laboratory strain S. pneumoniae R6 as recipient and chromosomal DNA of a high level penicillin resistant S. mitis B6 strain. After four transformation steps, alterations in five penicillin-binding proteins (PBP) were observed, and sequence analysis confirmed recombination events in the corresponding PBP genes. In order to detect regions where recombination with S. mitis DNA has occurred we analyzed the S. pneumoniae transformants by microarray analyses, using oligonucleotide microarrays designed for the S. pneumoniae genome and the S. mitis B6 genome as well.
Experiment types
comparative genomic hybridization by array, comparative genome hybridization, genetic modification, in vitro, individual genetic characteristics, strain or line
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3170.idf.txt
Sample and data relationshipE-MEXP-3170.sdrf.txt
Raw data (1)E-MEXP-3170.raw.1.zip
Array designsA-MEXP-1755.adf.txt, A-MEXP-1892.adf.txt
Links