E-MEXP-3078 - Divergent transcriptional response to thermal stress by Anopheles gambiae larvae carrying alternative arrangements of inversion 2La

Status
Released on 2 December 2011, last updated on 3 June 2014
Organism
Anopheles gambiae
Samples (45)
Array (1)
Protocols (6)
Description
shock treatment
Differential gene expression between the alternate 2La arrangements was examined for heat shock treated and control fourth instar larvae at three discrete time points to examine temporal variation in thermal stress response. One week after egg hatching, 72 fourth instar larvae of each 2La inversion polmorphism were randomly selected from multiple pans and placed into individual 13x100 mm glass culture tubes containing 2 mL of water. Heat shock treatment was carried out by placing tube racks containing larvae into a 38?C water bath for 60 min. After heat shock, larvae were placed back into 27?C water until collection, which took place 0.25 h, 2 h, and 8 h later by pooling 24 individuals into a 1.5 mL microcentrifuge tube and snap-freezing them in liquid nitrogen. Samples were stored at -80 ?C until RNA isolation. Control groups were treated identically for each time point with the only difference being that they were not exposed to the 60 min heat shock but rather placed into 27?C water
Experiment types
transcription profiling by array, array platform variation, co-expression, physiological process, time series
Contact
Citation
Divergent transcriptional response to thermal stress by Anopheles gambiae larvae carrying alternative arrangements of inversion 2La. Cassone BJ, Molloy MJ, Cheng C, Tan JC, Hahn MW, Besansky NJ. Mol Ecol 20(12):2567-2580 (2011), PMID:21535279
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3078.idf.txt
Sample and data relationshipE-MEXP-3078.sdrf.txt
Raw data (1)E-MEXP-3078.raw.1.zip
Processed data (1)E-MEXP-3078.processed.1.zip
Array designA-MEXP-1954.adf.txt