E-MEXP-307 - Transcription profiling of wheat GF- Transgenic _B1355-4-2 vs CTRL_Cadenza wheat lines endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination ?dpg)
Released on 5 October 2005, last updated on 2 May 2014
B1355-4-2 expresses five HMW subunits encoded by Glu-B1 (14, 15) and Glu D1 (5, 10) and transgene Glu-A1 (Ax1).Cadenza does not express Glu-A1 (Ax1. Line B1355-4-2(18) was generated by co-transformation with the ‘clean’ fragments of the HMW-GS 1Ax1 transgene (Halford, N.G. et al. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83, 373-378 (1992).)and the bar gene sequence. We compared the transcriptome of transgenic B1355-4-2(18) wheat line with their its background control-Cadenza bread wheat line. Transcriptomes comparisons were performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination –dpg). Each of the transcriptome comparisons analaysis was performed using three biological replicates (i.e. per line/tissue/developmental stage selected). Hybridisations were performed in reverse dye labelling.
transcription profiling by array, genetic modification
Maria M Baudo <email@example.com>, Keith K Edwards, Melloney M McCluskey, Michael M Holdsworth, Peter P Shewry, Rebecca R Lyons, Stephen S Powers