E-MEXP-3032 - Response of Streptomyces coelicolor to treatment with the antibiotics vancomycin bacitracin and moenomycin A

Status
Released on 29 April 2011, last updated on 18 January 2015
Organism
Streptomyces coelicolor A3(2)
Samples (43)
Array (1)
Protocols (6)
Description
To determine the response at the transcriptional level of S. coelicolor cells to treatment with 3 antibiotics that target distinct stages of cell wall biosynthesis, biological triplicate cultures of strain M600 were treated with sub-lethal concentrations (10 µg/ml) of vancomycin, bacitracin or moenomycin A. Samples were taken 0, 30, 60, 90 minutes after addition of the antibiotic. A negative control which received no drug treatment was also performed.
Experiment types
transcription profiling by array, co-expression, in vivo, stimulus or stress, time series
Contact
Citation
Genome-wide dynamics of a bacterial response to antibiotics that target the cell envelope. Hesketh, A., Hill, C., Mokhtar, J., Novotna, G., Bibb, M., and Hong, H.-J. BMC Genomics 12:226 (2011), Europe PMC 21569315
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-3032.idf.txt
Sample and data relationshipE-MEXP-3032.sdrf.txt
Raw data (1)E-MEXP-3032.raw.1.zip
Processed data (1)E-MEXP-3032.processed.1.zip
Array designA-AFFY-85.adf.txt
R ExpressionSetE-MEXP-3032.eSet.r
Links