Title: Affymetrix CEL analysis. Description:
nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
Title: Affymetrix Midi_Euk2v3 Hybridization. Description:
Drosophila larvae were grown on standard corn meal agar at 25 C.
(Parameters: time unit = seconds, temperature unit = C)
Raw data was normalized using ExpressionFileCreator module of GenePattern at Broad Institute. Quantile normalization with background correction was done following robust multi-array average (RMA) method.
Total RNA from wing imaginal discs of control and test genotypes were isolated using Qiagen RNeasy columns. Quality of RNA was ascertained using Agilent Bioanalyzer.
(Parameters: Extracted product = total_RNA, Amplification = none)