E-MEXP-271 - Transcription profiling of human hep2 cells infected with Streptococcus pyogenes over time

Status
Released on 30 June 2005, last updated on 2 May 2014
Organism
Homo sapiens
Samples (20)
Arrays (2)
Protocols (12)
Description
Two biological replicate experiments were performed to estimate the bias of the gene expression pattern of infected and non-infected HEp-2 cells. Microarrays hybridized with RNA from 2 h of non-infected HEp-2 cells were used as reference chips for the comparison with microarrays hybridized with RNA from 2 h and 4 h of eukaryotic cells exposed to wt-bacteria and .fasX-mutant. As a reference for chips hybridized with RNA prepared from 6 h p. i. and 8 h p. i. of both GAS-infected HEp-2 cells we used chips that were hybridized with RNA isolated from non-infected cells 8 h p. i. We also compared the microarray data from 2 h of non-infected HEp-2 cells with those from 8 h of non-infected HEp-2 cells to determine the influence of the extended culture on the non-infected cells. Only such genes which were differentially regulated after infection with wt-bacteria and .fasX-mutant infected cells and not differentially present in unequal amounts between the 2 h and 8 h of controls were included in the subsequent statistical analysis.
Experiment types
transcription profiling by array, stimulus or stress
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-271.idf.txt
Sample and data relationshipE-MEXP-271.sdrf.txt
Raw data (1)E-MEXP-271.raw.1.zip
Processed data (1)E-MEXP-271.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
R ExpressionSetE-MEXP-271.eSet.r
Links