E-MEXP-271 - Transcription profiling of human hep2 cells infected with Streptococcus pyogenes over time

Released on 30 June 2005, last updated on 2 May 2014
Homo sapiens
Samples (20)
Arrays (2)
Protocols (12)
Two biological replicate experiments were performed to estimate the bias of the gene expression pattern of infected and non-infected HEp-2 cells. Microarrays hybridized with RNA from 2 h of non-infected HEp-2 cells were used as reference chips for the comparison with microarrays hybridized with RNA from 2 h and 4 h of eukaryotic cells exposed to wt-bacteria and .fasX-mutant. As a reference for chips hybridized with RNA prepared from 6 h p. i. and 8 h p. i. of both GAS-infected HEp-2 cells we used chips that were hybridized with RNA isolated from non-infected cells 8 h p. i. We also compared the microarray data from 2 h of non-infected HEp-2 cells with those from 8 h of non-infected HEp-2 cells to determine the influence of the extended culture on the non-infected cells. Only such genes which were differentially regulated after infection with wt-bacteria and .fasX-mutant infected cells and not differentially present in unequal amounts between the 2 h and 8 h of controls were included in the subsequent statistical analysis.
Experiment types
transcription profiling by array, stimulus or stress
Investigation descriptionE-MEXP-271.idf.txt
Sample and data relationshipE-MEXP-271.sdrf.txt
Raw data (1)E-MEXP-271.raw.1.zip
Processed data (1)E-MEXP-271.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
R ExpressionSetE-MEXP-271.eSet.r