E-MEXP-2623 - Transcription profiling of human cell lines K562, Me45 and HCT116 with or without p53 knock-out to study the effect of ionizing radiation

Status
Released on 28 September 2010, last updated on 2 May 2014
Organism
Homo sapiens
Samples (16)
Arrays (2)
Protocols (7)
Description
The objective of the experiment was to compare changes in the transcriptome induced by direct X-irradiation of cells, using the human K562 erythroleukemic, Me45 melanoma and HCT116 colon cancer cell lines. The transcript levels of K542 and Me45 RNA samples were measured using Affymetrix HG-U133A microarrays and for HCT116 with HGU 133A 2.0 in time points 1, 12, 24 h after irradiation and compared to the levels of RNA from control cells. Experiments were repeated twice for Me45 and K562 cells.
Experiment types
transcription profiling by array, co-expression, in vitro, stimulus or stress, time series
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-2623.idf.txt
Sample and data relationshipE-MEXP-2623.sdrf.txt
Raw data (1)E-MEXP-2623.raw.1.zip
Processed data (1)E-MEXP-2623.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-37.adf.txt
R ExpressionSetE-MEXP-2623.eSet.r
Links