E-MEXP-2292 - Transcription profiling of honey bees for identification of changes in transcript abundance relating to Colony Collapse Disorder

Status
Submitted on 5 August 2009, released on 15 August 2009, last updated on 2 May 2014
Organism
Apis mellifera
Samples (15)
Array (1)
Protocols (6)
Description
Remaining adult bees in colonies suffering from CCD at apiaries in Florida, California and Pennsylvania were collected during the winter of 2006-2007. The health of CCD colonies was scored at the time of collection as either モsevereヤ or モmildヤ depending on the apparent strength of the colony. モHistoricalヤ bees, collected prior to the appearance of CCD and hence ostensibly healthy, were collected in 2004 and 2005 from colonies set up on new equipment and not receiving any miticide treatments from apiaries of The Pennsylvania State University near State College, PA. A combination looped, common-reference microarray design was used to compare historical and CCD samples with each other; a blend of RNA isolated from healthy colonies (collected near Urbana, IL in July 2007) served as reference. The microarray experiment compared the guts of bees from mildly and severely afflicted colonies collected in apiaries experiencing CCD on the East Coast (Florida and Pennsylvania) and West Coast (California) with a common reference.
Experiment types
transcription profiling by array, co-expression, organism status, reference
Contact
Citations
Changes in transcript abundance relating to Colony Collapse Disorder in honey bees (Apis mellifera).
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-2292.idf.txt
Sample and data relationshipE-MEXP-2292.sdrf.txt
Raw data (1)E-MEXP-2292.raw.1.zip
Processed data (1)E-MEXP-2292.processed.1.zip
Array designA-MEXP-755.adf.txt
R ExpressionSetE-MEXP-2292.eSet.r
Links