E-MEXP-2292 - Transcription profiling of honey bees for identification of changes in transcript abundance relating to Colony Collapse Disorder
Submitted on 5 August 2009, released on 15 August 2009, last updated on 2 May 2014
Remaining adult bees in colonies suffering from CCD at apiaries in Florida, California and Pennsylvania were collected during the winter of 2006-2007. The health of CCD colonies was scored at the time of collection as either ﾓsevereﾔ or ﾓmildﾔ depending on the apparent strength of the colony. ﾓHistoricalﾔ bees, collected prior to the appearance of CCD and hence ostensibly healthy, were collected in 2004 and 2005 from colonies set up on new equipment and not receiving any miticide treatments from apiaries of The Pennsylvania State University near State College, PA. A combination looped, common-reference microarray design was used to compare historical and CCD samples with each other; a blend of RNA isolated from healthy colonies (collected near Urbana, IL in July 2007) served as reference. The microarray experiment compared the guts of bees from mildly and severely afflicted colonies collected in apiaries experiencing CCD on the East Coast (Florida and Pennsylvania) and West Coast (California) with a common reference.
transcription profiling by array, co-expression, organism status, reference
Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera). Johnson RM, Evans JD, Robinson GE, Berenbaum MR. :14790-14795 (2009), Europe PMC 19706391
Changes in transcript abundance relating to Colony Collapse Disorder in honey bees (Apis mellifera).