E-MEXP-226 - Transcription profiling of human HUVEC cells with internalised S. aureus
Released on 31 October 2005, last updated on 2 May 2014
Staphylococcus aureus is a Gram-positive bacteria frequently isolated from patients with bloodstream infections. Endothelial cells (ECs) play an important role in host cell defence against bacteria, and recent reports have shown that infection of ECs with S. aureus induces a wide range of cytokines and cell surface receptors involved in activating the innate immune response (). The ability of S. aureus to invade nonphagocytic cells, including ECs, has been documented (), however, the knowledge of ECs role in pathogenesis of S. aureus infection is still limited.
In this study, we investigate the gene expression program initiated in human ECs by internalized S. aureus, using microarray analysis. We found 156 genes differentially regulated at least threefold, using arrays representing 14 239 genes. The main part of the upregulated genes code for cytokines, cell adhesion molecules, molecules involved in antigen presentation, cell signaling or cell metabolism. A variety of cytokines and chemokines seem to play an important role in S. aureus infection. Despite an apparent inflammatory response, internalized bacteria survived without inducing EC death.
transcription profiling by array, disease state, stimulus or stress