E-MEXP-215 - Transcription profiling of mesenchymal stem cells (MSC) maintained in FCS or autologous serum over time analysed at passage 4 and 10

Status
Released on 8 December 2004, last updated on 2 May 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (9)
Description
The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs)over time. To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for 2 different donors (i.e. donor3 and donor4). Likewise, we compared gene expression in MSCs maintained in regular growth medium supplemented with autologous serum(AS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for the same 2 donors (i.e. donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4 and at passage 10.
Experiment types
transcription profiling by array, growth condition
Contacts
Katrine B Fronsdal <katrine.fronsdal@labmed.uio.no>, Aboulghassem Shahdadfar, Finn P Reinholt, Jan E Brinchmann, Katrine Frønsdal, Siv H Tunheim
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-215.idf.txt
Sample and data relationshipE-MEXP-215.sdrf.txt
Raw data (1)E-MEXP-215.raw.1.zip
Processed data (1)E-MEXP-215.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-MEXP-215.eSet.r
Links