E-MEXP-215 - Transcription profiling of mesenchymal stem cells (MSC) maintained in FCS or autologous serum over time analysed at passage 4 and 10
Released on 8 December 2004, last updated on 2 May 2014
The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs)over time. To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for 2 different donors (i.e. donor3 and donor4). Likewise, we compared gene expression in MSCs maintained in regular growth medium supplemented with autologous serum(AS) for 10 passages with gene expression of MSCs cultured in the same conditions for 4 passages for the same 2 donors (i.e. donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4 and at passage 10.
transcription profiling by array, growth condition
Katrine B Fronsdal <email@example.com>, Aboulghassem Shahdadfar, Finn P Reinholt, Jan E Brinchmann, Katrine Frønsdal, Siv H Tunheim
In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability. Shahdadfar, Aboulghassem; Fronsdal, Katrine; Haug, Terje; Reinholt, Finn P.; Brinchmann, Jan E.