E-MEXP-214 - Transcription profiling of Mesenchymal stem cells (MSC) maintained in FCS vs autologous serum analysed at passage 4
Released on 8 December 2004, last updated on 2 May 2014
The purpose of this study was to determine whether the serum condition affected the gene expression in mesenchymal stem cells (MSCs). To that end, we compared gene expression in MSCs maintained in regular growth medium supplemented with fetal calf serum (FCS) with gene expression of MSCs cultured in regular growth medium supplemented with autologous serum (AS) for 3 different donors (i.e. donor2, donor3 and donor4). MSCs were cultured in FCS- or AS-supplemented medium and were analyzed at passage 4.
transcription profiling by array, growth condition
Katrine B Fronsdal <email@example.com>, Aboulghassem Shahdadfar, Finn P Reinholt, Jan E Brinchmann, Katrine Frønsdal, Siv H Tunheim
In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability. Shahdadfar, Aboulghassem; Fronsdal, Katrine; Haug, Terje; Reinholt, Finn P.; Brinchmann, Jan E.