E-MEXP-2000 - Transcription profiling of human monocyte-derived dendritic cells to compare the phenotypes of interferon-alpha and interleukin-4 conditioned dendritic cells

Status
Released on 8 January 2010, last updated on 12 October 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (6)
Description
The identification of molecules for apoptotic cells (AC) uptake by dendritic cells (DCs) and induction of T cell immunity is a major challenge in immunology.
DCs generated from monocytes in the presence of IFN-alpha (IFN-alpha-DCs) are competent in taking up ACs and inducing a strong T cell immunity. Capture of allogenetic apoptotic lymphocytes (APO-allo-PBLs) was associated with subcellular rearrangements of MHC class I/II molecules, subsequent cross-priming of auologous CD8+ T cells and CD4+ T cell activation. All these events were inhibited by the Scavenger Receptor (SR) LOX-1 protein, whose expression was up-regulated in IFN-alpha DCs. The results unravel a novel natural IFN-alpha-mediated pathway by which a defined SR molecule regulates AC uptake by DCs and subsequent induction of T cell immunity.
Experiment types
transcription profiling by array, cell type comparison, co-expression, in vitro
Contact
Citation
LOX-1 as a natural IFN-{alpha}-mediated signal for apoptotic cell uptake and antigen presentation in dendritic cells. Parlato, Stefania; Romagnoli, Giulia; Spadaro, Francesca; Canini, Irene; Sirabella, Paolo; Borghi, Paola; Ramoni, Carlo; Filesi, Ilaria; Biocca, Silvia; Gabriele, Lucia; Belardelli, Filippo. , PMID:20009034
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-2000.idf.txt
Sample and data relationshipE-MEXP-2000.sdrf.txt
Raw data (1)E-MEXP-2000.raw.1.zip
Processed data (1)E-MEXP-2000.processed.1.zip
Array designA-AFFY-33.adf.txt
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