E-MEXP-1953 - Transcription profiling of Sinorhizobium meliloti strain 1021FDC5 grown in liquid, solid or semi-solid media

Status
Released on 3 April 2009, last updated on 3 June 2014
Organism
Sinorhizobium meliloti
Samples (18)
Array (1)
Protocols (10)
Description
S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inoculation culture was added to 50 ml of fresh MM. At various times, samples were removed for determining viable cells counts as well as for RNA isolation/preparation (7 and 14 hours). For experiments on plates, 20 ml of MM containing 0.7% (Semisolid) or 1.3% (Solid) agar was dispensed onto each Petri dish and allowed to gel. The plates were air dried at room temperature for 15 min. 0.1 ml of the inoculation culture was plated onto the surface of the plates and allowed to dry for 10 min. The plates were then inverted and incubated at 30ºC.
Experiment types
 all pairs, co-expression, growth condition, time series, transcription profiling by array
Contact
Citation
Transcriptome profiling of a Sinorhizobium meliloti fadD mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming. Nogales J, Domínguez-Ferreras A, Amaya-Gómez CV, van Dillewijn P, Cuéllar V, Sanjuán J, Olivares J, Soto MJ. BMC Genomics 11:157 (2010), PMID:20210991
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1953.idf.txt
Sample and data relationshipE-MEXP-1953.sdrf.txt
Raw data (1)E-MEXP-1953.raw.1.zip
Array designA-MEXP-230.adf.txt
R ExpressionSetE-MEXP-1953.eSet.r