E-MEXP-1744 - Transcription profiling of human T cell leukaemia cell line Jurkat that were retrovirally transduced with constitutievely active forms of Notch to identify novel transcriptional targets of Notch signalling

Status
Released on 15 July 2009, last updated on 16 October 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
In this study we aimed to identify novel transcriptional targets of Notch signalling in the T cell leukaemia cell line, Jurkat. RNA was prepared from Jurkat cells retrovirally transduced with an empty vector (Mock) or vectors containing constitutively active forms of Notch (N1deltaE or N3deltaE), and used for Affymetrix microarray analysis. As expected, several known transcriptional target of Notch, such as HES1 and HERP2, were found to be overexpressed in Notch-transduced cells, however, many novel transcriptional targets of Notch signalling were identified using this approach. These included the T cell costimulatory molecule CD28, the anti-apoptotic protein GIMAP5, and inhibitor of DNA binding 1 (1D1).
Experiment types
transcription profiling by array, cellular process, co-expression, in vivo
Contact
Citation
Identification of novel Notch target genes in T cell leukaemia. Chadwick N, Zeef L, Portillo V, Fennessy C, Warrander F, Hoyle S, Buckle AM. , PMID:19508709
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1744.idf.txt
Sample and data relationshipE-MEXP-1744.sdrf.txt
Raw data (1)E-MEXP-1744.raw.1.zip
Processed data (1)E-MEXP-1744.processed.1.zip
Array designA-AFFY-33.adf.txt
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