E-MEXP-1712 - Transcription profiling of Streptococcus gordonii wildtype and Brf- deletion and insertion mutants

Status
Released on 1 August 2008, last updated on 2 May 2014
Organism
Streptococcus gordonii
Samples (6)
Array (1)
Protocols (6)
Description
RNA was isolated from early stationary phase S. gordonii V288(OD620 nm ~ 1.8).Bacteria were grown in chemically defined synthetic media (FMC) ( K2HPO4, 300 µg ml-1; KH2PO4 440 µg ml-1; Na2HPO4, 3.15 mg ml-1; NaH2PO4, 2.05 mg ml-1; (NH4)2SO4 0.6 mg ml-1; sodium citrate, 225 µg ml-1; riboflavin, 0.4 µg ml-1; biotin, 0.01 µg ml-1; folic acid, 0.1 µg ml-1; pantothenate, 0.8 µg ml-1; p-aminobenzoic acid 0.1 µg ml-1; thiamine 0.4 µg ml-1; nicotinamide 2.0 µg ml-1; pyridoxamine 0.8 µg ml-1; D-glucose 20 mg ml-1; sodium acetate 6 mg ml-1; adenine 35 µg ml-1; guanine µg ml-1; uracil µg ml-1; MgSO4, 200 µg ml-1; NaCl, 10 µg ml-1; FeSO4, 10 µg ml-1; MnSO4 10 µg ml-1; glutamine 5 µg ml-1; L-glutamic acid, 300 µg ml-1; L-valine, 100 µg ml-1; 110 µg ml-1 of L-lysine, L-asparate, L-isoleucine, L-methionine, L-serine, L-phenylalanine and 200 µg ml-1 of L-alanine, L-cystine, L-histidine, glycine, L-hydroxyproline, L-proline, L-tryptophan and L-tyrosine, pH 6.5) (Terleckyj & Shockman, 1975; Terleckyj et al., 1975) at 37°C in 5% CO2.
Experiment types
transcription profiling by array, co-expression, genetic modification, in vitro
Contact
Citation
The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1712.idf.txt
Sample and data relationshipE-MEXP-1712.sdrf.txt
Raw data (1)E-MEXP-1712.raw.1.zip
Processed data (1)E-MEXP-1712.processed.1.zip
Array designA-AFFY-125.adf.txt
R ExpressionSetE-MEXP-1712.eSet.r