E-MEXP-1683 - Transcription profiling of male zebra fish exposed to E2 and liver from non-vitellogenic and vitellogenic females to identify genes associated with vitellogenisis
Submitted on 9 August 2008, released on 26 May 2009, last updated on 2 May 2014
Two experiments were preformed, one for evaluating the effect of E2 exposure in males and a second experiment for comparing gene expression in the liver of non-vitellogenic females with that of vitellogenic females. The experiments designs were as follows:
1) Four months old zebrafish were divided into three groups consisting of 8 fish in each group: i) males (N=8) were exposed to E2 (Sigma-Aldrich, Israel) by immersion for 48 h (group E). The concentration used was 5 ?g/L (18 nM), as 3-4 ng/ml was determined to be the E2 natural concentration in the plasma of adult vitellogenic female ZF (Heiden et al 2006); ii) untreated males(N=8); iii) untreated vitellogenic females(N=8). Four replicate samples were prepared for each group and each replicate consisted of a pooled sample from livers of two fish.
2) In order to reveal the differences between vitellogenic and non-vitellogenic females, a second experiment was designed. One month old zebrafish were divided into two groups consisting of 32 fish in each group. Due to the small size of the liver, pooled samples from eight fish were prepared for each of the four replicates in the expression studies. The groups consisted of: i) fish that were kept under a light/dark cycle of 14/10 h for 5 weeks (N=32). with ovaries in vitellogenic stage; ii) fish that were kept under a light/dark cycle of 6/18 h for the same time period (N=32) with non-vitellogenic ovaries.
transcription profiling by array, co-expression, dose response, in vivo, physiological process, sex
Revealing genes associated with vitellogenesis in the liver of the zebrafish (Danio rerio) by transcriptome profiling. Levi L, Pekarski I, Gutman E, Fortina P, Hyslop T, Biran J, Levavi-Sivan B, Lubzens E. BMC Genomics 10:141 (2009)