E-MEXP-1402 - RNAi knock down of endogenous genes and luciferase reporters in human HeLa cells to examine the effect of seed complement frequency on off-target signatures

Status
Submitted on 4 January 2008, released on 15 March 2008, last updated on 2 May 2014
Organism
Homo sapiens
Array (1)
Protocols (7)
Description
siRNAs were designed against both endogenous genes and luciferase reporters to examine the effect of seed complement frequency on off-target signatures as measured by Agilent Human 1A gene expression analysis.
Experiment types
RNAi profiling by array, cellular modification, co-expression, compound treatment, in vitro, reference, self vs self
Contact
Citation
Experimental validation of the importance of seed complement frequency to siRNA specificity. Anderson, Emily M.; Birmingham, Amanda; Baskerville, Scott; Reynolds, Angela; Maksimova, Elena; Leake, Devin; Fedorov, Yuriy; Karpilow, Jon; Khvorova, Anastasia. RNA 14(5):853 (2008), Europe PMC 18367722
MIAME
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