E-MEXP-1335 - Transcription profiling by array of human intestinal mucosa after exposure to glutamine solution
Released on 13 November 2008, last updated on 9 October 2015
Glutamine mediates different aspects of gut physiology. Its effects on gene expression is, however, not known. To examine the effect of glutamine on intestinal gene expression in vivo in healthy subjects, an intestinal catheter was inserted into the proximal part of the small intestine, to enable injection of a glutamine-containing solution. Glutamine injection occurred over a 4-h period. After this period, mucosal tissue samples from the horizontal part of the duodenum were obtained by standard flexible gastroduodenoscopy, and Affymetrix microarrays were used to determine genome-wide gene expression profiles.
transcription profiling by array, co-expression, compound treatment, in vivo