E-MEXP-1315 - RNAi knock down of the DNA-repair enzyme and transcriptional co-activator APE1 in human HeLa cells
Released on 2 February 2009, last updated on 3 June 2014
Transcriptional profiling was done following APE1/Ref1 silencing using conditional gene expression knockdown by RNA interference (RNAi) technology in HeLa cell lines. APE1 is a DNA-repair enzyme and transcriptional co-activator. Knockdown leads to cell-growth arrest, apoptosis and impairment in the intracellular redox state and cytoskeletal perturbation.
transcription profiling by array, cellular modification, co-expression, in vivo
Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells. Vascotto C, Cesaratto L, Zeef LA, Deganuto M, DAmbrosio C, Scaloni A, Romanello M, Damante G, Taglialatela G, Delneri D, Kelley MR, Mitra S, Quadrifoglio F, Tell G. Proteomics 9(4):1058-1074 (2009), Europe PMC 19180539