E-MEXP-1315 - RNAi knock down of the DNA-repair enzyme and transcriptional co-activator APE1 in human HeLa cells

Status
Released on 2 February 2009, last updated on 3 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
Transcriptional profiling was done following APE1/Ref1 silencing using conditional gene expression knockdown by RNA interference (RNAi) technology in HeLa cell lines. APE1 is a DNA-repair enzyme and transcriptional co-activator. Knockdown leads to cell-growth arrest, apoptosis and impairment in the intracellular redox state and cytoskeletal perturbation.
Experiment types
transcription profiling by array, cellular modification, co-expression, in vivo
Contact
Citation
Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells. Vascotto C, Cesaratto L, Zeef LA, Deganuto M, DAmbrosio C, Scaloni A, Romanello M, Damante G, Taglialatela G, Delneri D, Kelley MR, Mitra S, Quadrifoglio F, Tell G. Proteomics 9(4):1058-1074 (2009), Europe PMC 19180539
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1315.idf.txt
Sample and data relationshipE-MEXP-1315.sdrf.txt
Raw data (1)E-MEXP-1315.raw.1.zip
Processed data (1)E-MEXP-1315.processed.1.zip
Array designA-AFFY-44.adf.txt
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