E-MEXP-1297 - Transcription profiling of Xenopus dorsal root ganglia conditioned in vitro vs freshly dissected control

Status
Submitted on 30 October 2007, released on 16 April 2008, last updated on 2 May 2014
Organism
Xenopus laevis
Samples (2)
Array (1)
Protocols (7)
Description
Axonal regeneration is enhanced by prior conditioning peripheral nerve lesions. Here we show that Xenopus dorsal root ganglia (DRGs) with attached peripheral nerves (PN-DRGs) can be conditioned in vitro, thereafter showing enhanced axonal growth in response to neurotrophins, similar to preparations conditioned by axotomy in vivo. In contrast to freshly dissected preparations, conditioned PN-DRGs show abundant neurotrophin-induced axonal growth in the presence of actinomycin D, suggesting synthesis of mRNA encoding proteins necessary for axonal elongation occurs during the conditioning period, and this was confirmed by oligonucleotide micro-array analysis.
Experiment types
transcription profiling by array, co-expression, growth condition, in vitro
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1297.idf.txt
Sample and data relationshipE-MEXP-1297.sdrf.txt
Raw data (1)E-MEXP-1297.raw.1.zip
Processed data (1)E-MEXP-1297.processed.1.zip
Array designA-AFFY-62.adf.txt
R ExpressionSetE-MEXP-1297.eSet.r
Links