E-MEXP-1227 - Transcription profiling by array of human umbilical vein endothelial cells exposed to pulsed or continuous estradiol

Status
Released on 29 August 2008, last updated on 9 October 2015
Organism
Homo sapiens
Samples (13)
Array (1)
Protocols (7)
Description
The experiment aims to investigate differences in the transcriptional response of human umbilical vein endothelial cells to estradiol treatment associated with the mode of administration. Human umbilical vein endothelial cells were treated for 5 days with a 1h pulse of 2.4 x 10-8 M 7β-estradiol (E2) once every day or with continuous 10-9M E2, ensuring cells received the same 24h E2 exposure (concentration x time) of two treatments. Control cells were treated with ethanol vehicle. For pulsed treatment, cells were washed and fresh medium was added after the 1h estradiol pulse. For continuous daily treatments, cells were incubated for 24h with media containing estradiol following the same scheme of media changes. The experiment was repeated at least three times.
Experiment types
transcription profiling by array, compound treatment design, stimulus or stress design
Contact
Citation
Differing transcriptional responses to pulsed or continuous estradiol exposure in human umbilical vein endothelial cells. Li J, Wang H, Johnson SM, Horner-Glister E, Thompson J, White IN, Al-Azzawi F.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1227.idf.txt
Sample and data relationshipE-MEXP-1227.sdrf.txt
Raw data (1)E-MEXP-1227.raw.1.zip
Processed data (1)E-MEXP-1227.processed.1.zip
Array designA-AFFY-33.adf.txt
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