E-MEXP-1227 - Transcription profiling by array of human umbilical vein endothelial cells exposed to pulsed or continuous estradiol
Released on 29 August 2008, last updated on 9 October 2015
The experiment aims to investigate differences in the transcriptional response of human umbilical vein endothelial cells to estradiol treatment associated with the mode of administration. Human umbilical vein endothelial cells were treated for 5 days with a 1h pulse of 2.4 x 10-8 M 7β-estradiol (E2) once every day or with continuous 10-9M E2, ensuring cells received the same 24h E2 exposure (concentration x time) of two treatments. Control cells were treated with ethanol vehicle. For pulsed treatment, cells were washed and fresh medium was added after the 1h estradiol pulse. For continuous daily treatments, cells were incubated for 24h with media containing estradiol following the same scheme of media changes. The experiment was repeated at least three times.
transcription profiling by array, compound treatment design, stimulus or stress design
Differing transcriptional responses to pulsed or continuous estradiol exposure in human umbilical vein endothelial cells. Li J, Wang H, Johnson SM, Horner-Glister E, Thompson J, White IN, Al-Azzawi F.