E-MEXP-1215 - Transcription profiling of yeast mutants Gal-YAH1, Gal-ATM1 and Gal-NBP35 to investigate responses to defects in cellular Fe-S protein maturation
Submitted on 17 August 2007, released on 14 December 2007, last updated on 2 May 2014
The biogenesis of iron-sulfur proteins in eukaryotes is an essential process involving the mitochondrial iron-sulfur cluster (ISC) assembly and export machineries and the cytosolic Fe/S protein assembly (CIA) apparatus. To define the integration of Fe/S protein biogenesis into cellular homeostasis, we compared the global transcriptional responses to defects in the three biogenesis systems in S. cerevisiae using DNA microarrays. Microarray analyses were carried out with regulatable yeast mutants in which representatives of each of the three biosynthetic systems could be depleted. In particular, we used the mutants Gal-YAH1, Gal-ATM1 and Gal-NBP35.
transcription profiling by array, co-expression, dye swap, genetic modification
Cellular and Mitochondrial Remodeling upon Defects in Iron-Sulfur Protein Biogenesis. Hausmann, Anja; Samans, Birgit; Lill, Roland; Muhlenhoff, Ulrich. J Biol Chem 283(13):8318 (2008), PMID:18227070