E-MEXP-1170 - Transcription profiling of wild type and sigma B mutant Liseteria monocytogenes to characterise the EGD-e sigma B regulon
Submitted on 9 July 2007, released on 2 July 2008, last updated on 2 May 2014
Briefly, over night cultures of L. monocytogenes EGD-e cells (wild-type and sigma B mutant) were grown in brain heart infusion (BHI) broth (Difco). Bacteria were diluted 1:50 in 20 ml fresh BHI and incubated using a 100 ml Erlenmeyer flask at 37 °C with shaking (180 rpm, Unitron [Infors]). Bacterial cells were harvested at OD600 1.0 (3 h), OD600 2.0 (4 h), OD600 2.5 (8 h) OD600 2.5 (16 h) using RNAprotect (Qiagen) according to manufacturer.
transcription profiling by array, co-expression, dye swap, genetic modification
Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon. Hain T, Hossain H, Chatterjee SS, Machata S, Volk U, Wagner S, Brors B, Haas S, Kuenne CT, Billion A, Otten S, Pane-Farre J, Engelmann S, Chakraborty T. BMC Microbiol 8:20 (2008), Europe PMC 18226246