E-MEXP-1106 - Transcription profiling of bark from Mexican Lime inoculated with two different isolates of the Citrus Tristeza Virus
Submitted on 10 May 2007, released on 18 May 2007, last updated on 2 May 2014
The transcriptional response of the Mexican Lime to two different isolates of the Citrus Tristeza Virus was evaluated. Virus Isolates were T305, which provokes severe symptoms in Lime plants, and isolate T385 which does not result in any visible symptoms. Five Lime plants were inoculated with each virus isolate, and four healthy plants were used to create a pooled control. Citrus custom arrays were hybridized with an infected sample and the pooled control, using a dyeswap design. In total, the experiment comprised hybridizations with twenty microarrays, ten for each virus isolate, including five biological replicates, each technically duplicated by dye-swapping.
transcription profiling by array, co-expression, disease state, dye swap
TRANSCRIPTIONAL RESPONSE OF CITRUS AURANTIFOLIA TO INFECTION BY CITRUS TRISTEZA VIRUS. Mónica Gandía, Ana Conesa, Gema Ancillo, José Gadea, Javier Forment Vicente Pallás, Ricardo Flores, Nuria Duran-Vila, Pedro Moreno and José Guerri. Virology (2007)