E-MEXP-1078 - Transcription profiling by array of human HeLa cells treated with the major listerial toxin listeriolysin
Released on 1 April 2008, last updated on 13 May 2014
Upon infection, pathogens reprogram host gene expression. In eukaryotic cells, genetic reprogramming is induced by the concerted activation/repression of transcription factors and various histone modifications that control DNA accessibility in chromatin. We report here that the bacterial pathogen, Listeria monocytogenes, induces a dramatic dephosphorylation of histone H3 as well as a deacetylation of histone H4 during early phases of infection. This effect is mediated by the major listerial toxin listeriolysin (LLO), in a pore forming independent manner. Strikingly, a similar effect is also observed with other toxins of the same family, such as Clostridium perfringens perfringolysin (PFO) and Streptococcus pneumoniae pneumolysin (PLY). The decreased levels of histone modifications correlate with a reduced transcriptional activity of a subset of host genes, including key immunity genes. Thus, manipulation of the epigenetic information emerges here as an unsuspected function shared by several bacterial toxins, highlighting a common strategy used by intracellular and extracellular pathogens to modulate the host response early during infection.
transcription profiling by array, co-expression, compound treatment design, in vitro
Histone modifications induced by a family of bacterial toxins. Hamon MA, Batsché E, Régnault B, Tham TN, Seveau S, Muchardt C, Cossart P. , Europe PMC 17675409